Deciphering the mechanisms of tumorigenesis in human pancreatic ductal epithelial cells.

ABSTRACT:

Purpose

The most common genetic lesions in pancreatic ductal adenocarcinoma (PDAC) have been identified. However, significant gaps still exist in our understanding of how such genetic alterations act in concert to induce PDAC development. In this study, we investigated the mechanism of tumorigenic transformation in the immortalized human pancreatic ductal epithelial (HPDE) cell line by sequentially introducing PDAC signature alterations into this cell line.

Experimental design

The phenotype for stable expression of mutant K-ras, Her2, p16/p14shRNA, and Smad4shRNA in HPDE cells was examined by assays for cell proliferation, migration, invasion, soft agar, and orthotopic tumorigenesis. The mechanisms of tumorigenic transformation were further explored by gene expression profil

SUBMITTER: Chang Z 

PROVIDER: S-EPMC3576034 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature