Proteomic analysis of the effects of exogenous calcium on hypoxic-responsive proteins in cucumber roots.

ABSTRACT:

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Background

Hypoxia acts as a plant stress factor, particularly in cucumbers plants under hydroponic culture. Calcium is involved in stress signal transmission and in the growth of plants. To determine the effect of exogenous calcium on hypoxic-responsive proteins in cucumber (Cucumis sativus L. cv. Jinchun No.2) roots, proteomic analysis was performed using two-dimensional electrophoresis (2-DE) and mass spectrometry.

Results

Cucumber roots were used to analyze the influence of hypoxia on plants. The expressions of 38 protein spots corresponding to enzymes were shown to change in response to hypoxia. Of these, 30 spots were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF/TOF MS analysis). The proteins w

SUBMITTER: He L 

PROVIDER: S-EPMC3576256 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature