Chemo-enzymatic synthesis and in vitro cytokine profiling of tailor-made oligofructosides.

ABSTRACT:

Background

It is well known that carbohydrates play fundamental roles in cell signaling and infection processes as well as tumor formation and progression. However, the interaction pathways and cellular receptors targeted by carbohydrates and glycoconjugates remain poorly examined and understood. This lack of research stems, at least to a major part, from accessibility problems of large, branched oligosaccharides.

Results

To test glycan-cell interactions in vitro, a variety of tailored oligosaccharides was synthesized chemo-enzymatically. Glycosyltransferases from the GRAS organisms Bacillus megaterium (SacB) and Aspergillus niger (Suc1) were used in this study. Substrate engineering of these glycosyltransferases generally acting on sucrose leads to the controlled formation

SUBMITTER: Homann A 

PROVIDER: S-EPMC3576278 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature