Dual RMCE for efficient re-engineering of mouse mutant alleles.

ABSTRACT: We have developed dual recombinase-mediated cassette exchange (dRMCE) to efficiently re-engineer the thousands of available conditional alleles in mouse embryonic stem cells. dRMCE takes advantage of the wild-type loxP and FRT sites present in these conditional alleles and in many gene-trap lines. dRMCE is a scalable, flexible tool to introduce tags, reporters and mutant coding regions into an endogenous locus of interest in an easy and highly efficient manner.

SUBMITTER: Osterwalder M 

PROVIDER: S-EPMC3576631 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature