Corticosteroid insensitivity of chemokine expression in airway smooth muscle of patients with severe asthma.

ABSTRACT:

Background

Patients with severe asthma are less responsive to the beneficial effects of corticosteroid therapy.

Objective

We investigated whether corticosteroid insensitivity was present in airway smooth muscle cells (ASMCs) of patients with severe asthma.

Methods

ASMCs cultured from bronchial biopsy specimens of nonasthmatic control subjects (n = 12) and patients with nonsevere (n = 10) or severe (n = 10) asthma were compared for the effect of dexamethasone on suppression of TNF-α- and IFN-γ-induced CCL11 (eotaxin), CXCL8 (IL-8), and CX3CL1 (fractalkine) expression. The mechanisms of corticosteroid insensitivity are also determined.

Results

CCL11 release was higher in ASMCs of patients with nonsevere but not severe asthma and nonasthmatic control subjects; CXC

SUBMITTER: Chang PJ 

PROVIDER: S-EPMC3578224 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature