Farnesoid X receptor protects hepatocytes from injury by repressing miR-199a-3p, which increases levels of LKB1.

ABSTRACT:

Background & aims

Hepatocyte injury occurs during liver fibrogenesis. MicroRNAs (miRNA) regulate some of these processes, and some are regulated by the farnesoid X receptor (FXR). We investigated the effect of repression of specific miRNAs by FXR in hepatocyte injury using fibrotic liver tissue from patients and hepatocytes.

Methods

We used immunohistochemistry or real-time polymerase chain reaction to analyze proteins and miRNAs in human and mouse liver samples. HepG2 cells were transfected with pre-miRNA, antisense oligonucleotides, small interfering RNAs, the 3'-untranslated region of liver kinase B1 (LKB1) (STK11), or constructs for overexpression, and analyzed.

Results

Liver tissue from patients with severe fibrosis had lower levels of FXR and greater amounts of

SUBMITTER: Lee CG 

PROVIDER: S-EPMC3578415 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature