The DNA repair protein ALKBH2 mediates temozolomide resistance in human glioblastoma cells.

ABSTRACT:

Introduction

Glioblastoma multiforme (GBM; World Health Organization astrocytoma grade IV) is the most frequent and most malignant primary brain tumor in adults. Despite multimodal therapy, all such tumors practically recur during the course of therapy, causing a median survival of only 14.6 months in patients with newly diagnosed GBM. The present study was aimed at examining the expression of the DNA repair protein AlkB homolog 2 (ALKBH2) in human GBM and determining whether it could promote resistance to temozolomide chemotherapy.

Methods

ALKBH2 expression in GBM cell lines and in human GBM was determined by quantitative real-time PCR (qRT-PCR) and gene expression analysis, respectively. Drug sensitivity was assessed in GBM cells overexpressing ALKBH2 and in cells in which

SUBMITTER: Johannessen TC 

PROVIDER: S-EPMC3578482 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature