Molecular mapping to species level of the tonsillar crypt microbiota associated with health and recurrent tonsillitis.

ABSTRACT: The human palatine tonsils, which belong to the central antigen handling sites of the mucosal immune system, are frequently affected by acute and recurrent infections. This study compared the microbiota of the tonsillar crypts in children and adults affected by recurrent tonsillitis with that of healthy adults and children with tonsillar hyperplasia. An in-depth 16S rRNA gene based pyrosequencing approach combined with a novel strategy that included phylogenetic analysis and detection of species-specific sequence signatures enabled identification of the major part of the microbiota to species level. A complex microbiota consisting of between 42 and 110 taxa was demonstrated in both children and adults. This included a core microbiome of 12 abundant genera found in all samples regardless of

SUBMITTER: Jensen A 

PROVIDER: S-EPMC3578847 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature