Differential microRNA regulation correlates with alternative polyadenylation pattern between breast cancer and normal cells.

ABSTRACT: Alternative polyadenylation (APA) could result in mRNA isoforms with variable lengths of 3' UTRs. Gain of microRNA target sites in the 3' UTR of a long mRNA isoform may cause different regulation from the corresponding short isoform. It has been known that cancer cells globally exhibit a lower ratio of long and short isoforms (LSR); that is, they tend to express larger amounts of short isoforms. The objective of this study is to illustrate the relationship between microRNA differential regulation and LSR. We retrieved public APA annotations and isoform expression profiles of breast cancer and normal cells from a high-throughput sequencing method study specific for the mRNA 3' end. Combining microRNA expression profiles, we performed statistical analysis to reveal and estimate microRNA regu

SUBMITTER: Liaw HH 

PROVIDER: S-EPMC3578872 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature