An inducible translocation strategy to rapidly activate and inhibit small GTPase signaling pathways.

ABSTRACT: We made substantial advances in the implementation of a rapamycin-triggered heterodimerization strategy. Using molecular engineering of different targeting and enzymatic fusion constructs and a new rapamycin analog, Rho GTPases were directly activated or inactivated on a timescale of seconds, which was followed by pronounced cell morphological changes. As signaling processes often occur within minutes, such rapid perturbations provide a powerful tool to investigate the role, selectivity and timing of Rho GTPase-mediated signaling processes.

SUBMITTER: Inoue T 

PROVIDER: S-EPMC3579513 | biostudies-literature | 2005 Jun

REPOSITORIES: biostudies-literature