Molecular cloning and expression analyses of porcine MAP1LC3A in the granulosa cells of normal and miniature pig.

ABSTRACT:

Background

The members of the microtubule-associated protein 1 light chain (MAP1LC) family, especially those of the LC3 family (MAP1LC3A, B, C), are known to induce autophagy upon localization onto the autophagosomal membrane. In this regard, LC3 can be utilized as a marker for the formation of autophagosomes during the process of autophagy. The aims of this study are to clone porcine MAP1LC3A, and analyze the pattern of its expression in the ovarian tissues of normal and miniature pig ovary in an attempt to understand the distinct mode of apoptosis between two strains.

Methods

Rapid amplification of cDNA ends (RACE) were used to obtain the 5' and 3' ends of the porcine MAP1LC3A full length cDNA. Reverse-transcriptase-PCR (RT-PCR), real-time PCR, and western blot analysis we

SUBMITTER: Kim SH 

PROVIDER: S-EPMC3579721 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature