CpG island hypermethylation frequently silences FILIP1L isoform 2 expression in prostate cancer.

ABSTRACT:

Purpose

Senescence related regulatory pathways serve as barriers to cancer immortalization and progression but they are currently not well defined. FILIP1L is a growth inhibitory gene with multiple isoforms whose expression is increased in senescent prostate and prostate cancer cells, and decreased in many cancers. We investigated whether DNA methylation regulates FILIP1L in senescence and in prostate cancer development.

Materials and methods

FILIP1L mRNA expression was assessed in prostate cancer and associated normal prostate tissues using quantitative polymerase chain reaction. A tissue microarray was constructed using 95 prostate cancer specimens and 45 benign prostate specimens. Vectra™ imaging was used to quantitate nuclear and cytoplasmic FILIP1L protein expression. B

SUBMITTER: Desotelle J 

PROVIDER: S-EPMC3581042 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature