Hepatocyte nuclear factor 4alpha is essential for embryonic development of the mouse colon.

ABSTRACT:

Background & aims

Hepatocyte nuclear factor 4 alpha (HNF4alpha) is a transcription factor that has been shown to be required for hepatocyte differentiation and development of the liver. It has also been implicated in regulating expression of genes that act in the epithelium of the lower gastrointestinal tract. This implied that HNF4alpha might be required for development of the gut.

Methods

Mouse embryos were generated in which Hnf4a was ablated in the epithelial cells of the fetal colon by using Cre-loxP technology. Embryos were examined by using a combination of histology, immunohistochemistry, DNA microarray, reverse-transcription polymerase chain reaction, electrophoretic mobility shift assays, and chromatin immunoprecipitation analyses to define the consequences of loss

SUBMITTER: Garrison WD 

PROVIDER: S-EPMC3581272 | biostudies-literature | 2006 Apr

REPOSITORIES: biostudies-literature