Templating effect in DNA proximity ligation enables use of non-bioorthogonal chemistry in biological fluids.

ABSTRACT: Here we describe the first example of selective reductive amination in biological fluids using split aptamer proximity ligation (StAPL). Utilizing the cocaine split aptamer, we demonstrate small-molecule-dependent ligation that is dose-dependent over a wide range of target concentrations in buffer, human blood serum and artificial urine medium. We explore the substrate binding preferences of the split aptamer and find that the cinchona alkaloids quinine and quinidine bind to the aptamer with higher affinity than cocaine. This increased affinity leads to improved detection limits for these small-molecule targets. We also demonstrate that linker length and hydrophobicity impact the efficiency of split aptamer ligation. The ability to carry out selective chemical transformations using non-bio

SUBMITTER: Spiropulos NG 

PROVIDER: S-EPMC3581511 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature