Spatial patterning of endothelium modulates cell morphology, adhesiveness and transcriptional signature.

ABSTRACT: Microscale and nanoscale structures can spatially pattern endothelial cells (ECs) into parallel-aligned organization, mimicking their cellular alignment in blood vessels exposed to laminar shear stress. However, the effects of spatial patterning on the function and global transcriptome of ECs are incompletely characterized. We used both parallel-aligned micropatterned and nanopatterned biomaterials to evaluate the effects of spatial patterning on the phenotype of ECs, based on gene expression profiling, functional characterization of monocyte adhesion, and quantification of cellular morphology. We demonstrate that both micropatterned and aligned nanofibrillar biomaterials could effectively guide EC organization along the direction of the micropatterned channels or nanofibrils, respectively

SUBMITTER: Huang NF 

PROVIDER: S-EPMC3581686 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature