Pseudomonas fluorescens-like bacteria from the stomach: a microbiological and molecular study.

ABSTRACT:

Aim

To characterize oxidase- and urease-producing bacterial isolates, grown aerobically, that originated from antral biopsies of patients suffering from acid peptic diseases.

Methods

A total of 258 antral biopsy specimens were subjected to isolation of bacteria followed by tests for oxidase and urease production, acid tolerance and aerobic growth. The selected isolates were further characterized by molecular techniques viz. amplifications for 16S rRNA using universal eubacterial and HSP60 gene specific primers. The amplicons were subjected to restriction analysis and partial sequencing. A phylogenetic tree was generated using unweighted pair group method with arithmetic mean (UPGMA) from evolutionary distance computed with bootstrap test of phylogeny. Assessment of acidity t

SUBMITTER: Patel SK 

PROVIDER: S-EPMC3581993 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature