Regulation of integrin trafficking, cell adhesion, and cell migration by WASH and the Arp2/3 complex.

ABSTRACT: WASH is a nucleation-promoting factor for the Arp2/3 complex that is implicated in multiple endocytic trafficking pathways including receptor recycling, cargo degradation, and retromer-mediated receptor retrieval. We sought to examine whether WASH plays an important role in trafficking of specialized cargo molecules such as integrins, for which trafficking is highly regulated during cell migration. We observed that subdomains of early/sorting endosomes associated with dynamic WASH and filamentous actin, and α5-integrins trafficked through this population of endosomes. Depletion of WASH caused accumulation of α5-integrins in intracellular compartments, reduction of α5-integrin localization at focal adhesions, and reduction in focal adhesion number. Transport of α5-integrins from internal en

SUBMITTER: Duleh SN 

PROVIDER: S-EPMC3582321 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature