Predicting isoelectric points of nonfunctional mitochondria from Monte Carlo simulations of surface compositions.

ABSTRACT: Mitochondria are heterogeneous organelles involved in energy production, metabolism, and cellular signaling that oftentimes are isolated from cells for chemical characterization (e.g., proteomic analysis). The chemical composition of the mitochondrial outer membrane is one of the factors defining the mitochondrial isoelectric point (pI), which is a property useful for the analysis and characterization of isolated mitochondria. We previously used capillary isoelectric focusing (cIEF) with laser-induced-fluorescence detection to determine the experimental pI of individual mitochondria after their isolation under depolarizing conditions. This technique revealed that, when kept nonfunctional, mitochondrial pI is heterogeneous as displayed by the observed distributions of pI. To model the effec

SUBMITTER: Wolken GG 

PROVIDER: S-EPMC3582746 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature