Reannotation of translational start sites in the genome of Mycobacterium tuberculosis.

ABSTRACT: Identification and correction of incorrect ORF start sites is important for a variety of experimental and analytical purposes, ranging from cloning to inference of operon structure. The genome of the H37Rv reference strain of Mycobacterium tuberculosis (Mtb) was originally annotated when it was first sequenced nearly 15 years ago. While this annotation has served the TB research community well as a standard of reference for over a decade, it has been demonstrated experimentally that the actual start sites for an estimated 5-10% of open reading frames differ from the annotation. In this paper, we present a comprehensive bioinformatic analysis of all 3989 ORFs (open reading frames) in the M. tuberculosis H37Rv genome. Our method combines information from comparative analysis (alignment to st

SUBMITTER: DeJesus MA 

PROVIDER: S-EPMC3582765 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature