Suppression of FUT1 attenuates cell proliferation in the HER2-overexpressing cancer cell line NCI-N87.

ABSTRACT: Lewis Y (LeY) antigen is an oligosaccharide that is highly expressed at the cell surface in various human cancers. Increased LeY expression activates epidermal growth factor receptor (EGFR) and human epidermal growth factor receptor 2 (HER2) and promotes cell proliferation in EGFR-overexpressing cells. However, the effect of downregulation of LeY expression on cell proliferation in HER2-overexpressing cells remains unknown. FUT1 encodes α1,2-fucosyltransferase, a key enzyme for LeY synthesis. We knocked down FUT1 by short interfering RNA (siRNA) in four HER2-overexpressing human cancer cell lines, including NCI-N87, MKN7, SKBr3 and BT474. We investigated whether downregulation of LeY and alterat

SUBMITTER: Kawai S 

PROVIDER: S-EPMC3583482 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature