Advances in plant gene-targeted and functional markers: a review.
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ABSTRACT: Public genomic databases have provided new directions for molecular marker development and initiated a shift in the types of PCR-based techniques commonly used in plant science. Alongside commonly used arbitrarily amplified DNA markers, other methods have been developed. Targeted fingerprinting marker techniques are based on the well-established practices of arbitrarily amplified DNA methods, but employ novel methodological innovations such as the incorporation of gene or promoter elements in the primers. These markers provide good reproducibility and increased resolution by the concurrent incidence of dominant and co-dominant bands. Despite their promising features, these semi-random markers suffer from possible problems of collision and non-homology analogous to those found with randomly
SUBMITTER: Poczai P
PROVIDER: S-EPMC3583794 | biostudies-literature | 2013 Feb
REPOSITORIES: biostudies-literature
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