The mechanism of nucleotide excision repair-mediated UV-induced mutagenesis in nonproliferating cells.

ABSTRACT: Following the irradiation of nondividing yeast cells with ultraviolet (UV) light, most induced mutations are inherited by both daughter cells, indicating that complementary changes are introduced into both strands of duplex DNA prior to replication. Early analyses demonstrated that such two-strand mutations depend on functional nucleotide excision repair (NER), but the molecular mechanism of this unique type of mutagenesis has not been further explored. In the experiments reported here, an ade2 adeX colony-color system was used to examine the genetic control of UV-induced mutagenesis in nondividing cultures of Saccharomyces cerevisiae. We confirmed a strong suppression of two-strand mutagenesis in NER-deficient backgrounds and demonstrated that neither mismatch repair nor interstrand cross

SUBMITTER: Kozmin SG 

PROVIDER: S-EPMC3583999 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature