Highly efficient and selective enrichment of peptide subsets combining fluorous chemistry with reversed-phase chromatography.

ABSTRACT: The selective capture of target peptides poses a great challenge to modern chemists and biologists, especially when enriching them from proteome samples possessing extremes in concentration dynamic range and sequence diversity. While approaches based on traditional techniques such as biotin-avidin pairing offer versatile tools to design strategies for selective enrichment, problems are still encountered due to sample loss or poor selectivity of enrichment. Here we show that the recently introduced fluorous chemistry approach has attractive properties as an alternative method for selective enrichment. Through appending a perfluorine group to the target peptide, it is possible to dramatically increase the peptide's hydrophobicity and thus enable facile separation of labeled from non-labeled

SUBMITTER: Ying W 

PROVIDER: S-EPMC3584324 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature