Cyclin D1 degradation and p21 induction contribute to growth inhibition of colorectal cancer cells induced by epigallocatechin-3-gallate.

ABSTRACT:

Purpose

The public has paid attention to green tea due to its health benefits. Epigallocatechin-3-gallate (EGCG), the major component of green tea, is well documented to induce apoptosis and cell cycle arrest in cancer cells by targeting multiple signal transduction pathways. However, the detailed mechanism(s) of action needs to be determined.

Methods

Cell growth was evaluated by MTT assay, cell cycle analysis, and caspase 3/7 activity. Protein expression was analyzed through Western blotting. Reverse transcription polymerase chain reaction was used for examining mRNA expression of p21 and cyclin D1. The promoter activity of p21 was assessed by the luciferase reporter system.

Results

We identified cyclin D1 and p21 as molecular targets of EGCG in human colorectal canc

SUBMITTER: Zhang X 

PROVIDER: S-EPMC3584327 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature