Evaluation of suitable reference genes for gene expression studies in porcine PBMCs in response to LPS and LTA.

ABSTRACT:

Background

As an in vitro model porcine peripheral blood mononuclear cells (PBMCs) is frequently used as for immunogenetic research with the stimulation of bacterial antigens. To investigate the immunocompetence of PBMCs for recognition of Gram-positive and Gram-negative bacteria and in order to dissect the pathogenesis of diseases, gene expression assay is most commonly used. The gene expressions are required to normalize for reference genes which have tremendous effect on the results of expression study. The reference genes should be stably expressed between different cells under a variety of experimental conditions, but recent influx of data showed that expression stability of reference genes are varied under different experimental conditions. But data regarding the expression s

SUBMITTER: Cinar MU 

PROVIDER: S-EPMC3584940 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature