Faster protein splicing with the Nostoc punctiforme DnaE intein using non-native extein residues.

ABSTRACT: Inteins are naturally occurring intervening sequences that catalyze a protein splicing reaction resulting in intein excision and concatenation of the flanking polypeptides (exteins) with a native peptide bond. Inteins display a diversity of catalytic mechanisms within a highly conserved fold that is shared with hedgehog autoprocessing proteins. The unusual chemistry of inteins has afforded powerful biotechnology tools for controlling enzyme function upon splicing and allowing peptides of different origins to be coupled in a specific, time-defined manner. The extein sequences immediately flanking the intein affect splicing and can be defined as the intein substrate. Because of the enormous potential complexity of all possible flanking sequences, studying intein substrate specificity has bee

SUBMITTER: Cheriyan M 

PROVIDER: S-EPMC3585056 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature