Effects of flame made zinc oxide particles in human lung cells - a comparison of aerosol and suspension exposures.

ABSTRACT:

Background

Predominantly, studies of nanoparticle (NPs) toxicology in vitro are based upon the exposure of submerged cell cultures to particle suspensions. Such an approach however, does not reflect particle inhalation. As a more realistic simulation of such a scenario, efforts were made towards direct delivery of aerosols to air-liquid-interface cultivated cell cultures by the use of aerosol exposure systems.This study aims to provide a direct comparison of the effects of zinc oxide (ZnO) NPs when delivered as either an aerosol, or in suspension to a triple cell co-culture model of the epithelial airway barrier. To ensure dose-equivalence, ZnO-deposition was determined in each exposure scenario by atomic absorption spectroscopy. Biological endpoints being investigated after 4 or 2

SUBMITTER: Raemy DO 

PROVIDER: S-EPMC3585858 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature