Unrestrictive identification of post-translational modifications in the urine proteome without enrichment.

ABSTRACT:

Background

Research on the human urine proteome may lay the foundation for the discovery of relevant disease biomarkers. Post-translational modifications (PTMs) have important effects on the functions of protein biomarkers. Identifying PTMs without enrichment adds no extra steps to conventional identification procedures for urine proteomics. The only difference is that this method requires software that can conduct unrestrictive identifications of PTMs. In this study, routine urine proteomics techniques were used to identify urine proteins. Unspecified PTMs were searched by MODa and PEAKS 6 automated software, followed by a manual search to screen out in vivo PTMs by removing all in vitro PTMs and amino acid substitutions.

Results

There were 75 peptides with 6 in vivo PTMs t

SUBMITTER: Liu L 

PROVIDER: S-EPMC3585864 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature