Thermomechanical analysis of freezing-induced cell-fluid-matrix interactions in engineered tissues.

ABSTRACT: Successful cryopreservation of functional engineered tissues (ETs) is significant to tissue engineering and regenerative medicine, but it is extremely challenging to develop a successful protocol because the effects of cryopreservation parameters on the post-thaw functionality of ETs are not well understood. Particularly, the effects on the microstructure of their extracellular matrix (ECM) have not been well studied, which determines many functional properties of the ETs. In this study, we investigated the effects of two key cryopreservation parameters--(i) freezing temperature and corresponding cooling rate; and (ii) the concentration of cryoprotective agent (CPA) on the ECM microstructure as well as the cellular viability. Using dermal equivalent as a model ET and DMSO as a model CPA, f

SUBMITTER: Han B 

PROVIDER: S-EPMC3586257 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature