Diagnostic performance of two highly multiplexed respiratory virus assays in a pediatric cohort.

ABSTRACT:

Background

Rapid detection of respiratory viruses is important for management and infection control in hospitalized patients. Multiplex nucleic acid tests (NATs) have begun to replace conventional methods as gold standards for respiratory virus detection.

Objective

To compare the performance of two large multiplex NATS, ResPlex II (RPII) and Respiratory Virus Surveillance kit with electrospray ionization mass spectrometry (RVS/MS) using nasopharyngeal aspirates (NPAs) from hospitalized children who had been tested previously with conventional methods.

Study design

Stored residual NPAs (N=306) were tested concomitantly by RPII and RVS/MS. Alternate NATs were used to adjudicate discordant results.

Results

More viruses were detected with multiplex NATs (RPII, 110;

SUBMITTER: Forman MS 

PROVIDER: S-EPMC3586269 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature