Subcellular spatial segregation of integrin subtypes by patterned multicomponent surfaces.

ABSTRACT: While it is well known that individual integrins are critical mediators of cell behavior, recent work has shown that when multiple types of integrins simultaneously engage the ECM, cell functions are enhanced. However, it is not known how integrins spatially coordinate to regulate cell adhesion because no reliable method exists to segregate integrins on the cell membrane. Here, we use a microcontact printing-based strategy to pattern multiple ECMs that bind distinct integrins in order to study how integrins might interact. In our technique, proteins are first adsorbed uniformly to a poly(dimethyl siloxane) stamp, and then selectively "de-inked." Our strategy overcomes several inherent limitations of conventional microcontact printing, including stamp collapse and limited functionality of t

SUBMITTER: Desai RA 

PROVIDER: S-EPMC3586560 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature