Adhesion regulates MAP kinase/ternary complex factor exchange to control a proliferative transcriptional switch.

ABSTRACT:

Background

The ternary complex factors (TCFs; Elk1, Net, and Sap-1) are growth factor-responsive transcription cofactors of serum response factor (SRF) and are activated by MAP kinase (MAPK) phosphorylation to regulate immediate early gene transcription. Although cell adhesion also can regulate immediate early genes and proliferation, the mechanism for this effect has remained unexplored.

Results

Restricting adhesion and spreading of G(0)-synchronized cells on substrates with decreasing size of micropatterned islands of fibronectin suppressed serum-induced immediate early gene expression and S phase entry. Knockdown of Sap-1 decreased expression of the immediate early genes egr1 and fos and subsequent proliferation normally present with high adhesion, whereas knockdown of Ne

SUBMITTER: Wozniak MA 

PROVIDER: S-EPMC3586562 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature