Correlative live-cell and superresolution microscopy reveals cargo transport dynamics at microtubule intersections.

ABSTRACT: Intracellular transport plays an essential role in maintaining the organization of polarized cells. Motor proteins tether and move cargos along microtubules during long-range transport to deliver them to their proper location of function. To reach their destination, cargo-bound motors must overcome barriers to their forward motion such as intersection points between microtubules. The ability to visualize how motors navigate these barriers can give important information about the mechanisms that lead to efficient transport. Here, we first develop an all-optical correlative imaging method based on single-particle tracking and superresolution microscopy to map the transport trajectories of cargos to individual microtubules with high spatiotemporal resolution. We then use this method to study

SUBMITTER: Balint S 

PROVIDER: S-EPMC3587250 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature