Hexamethylene diisocyanate (HDI) vapor reactivity with glutathione and subsequent transfer to human albumin.

ABSTRACT:

Introduction

Airway fluid glutathione (GSH) reactivity with inhaled vapors of diisocyanate, a common occupational allergen, is postulated to be a key step in exposure-induced asthma pathogenesis.

Methods

A mixed (vapor/liquid) phase exposure system was used to model the in vivo reactivity of inhaled HDI vapor with GSH in the airway fluid. HDI-GSH reaction products, and their capacity to transfer HDI to human albumin, were characterized through mass spectrometry and serologic assays, using HDI-specific polyclonal rabbit serum.

Results

HDI vapor exposure of 10mM GSH solutions resulted in primarily S-linked, bis(GSH)-HDI reaction products. In contrast, lower GSH concentrations (100μM) resulted in mainly mono(GSH)-HDI conjugates, with varying degrees of HDI hydrolysis, di

SUBMITTER: Wisnewski AV 

PROVIDER: S-EPMC3587365 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature