Genome wide allele frequency fingerprints (GWAFFs) of populations via genotyping by sequencing.

ABSTRACT: Genotyping-by-Sequencing (GBS) is an excellent tool for characterising genetic variation between plant genomes. To date, its use has been reported only for genotyping of single individuals. However, there are many applications where resolving allele frequencies within populations on a genome-wide scale would be very powerful, examples include the breeding of outbreeding species, varietal protection in outbreeding species, monitoring changes in population allele frequencies. This motivated us to test the potential to use GBS to evaluate allele frequencies within populations. Perennial ryegrass is an outbreeding species, and breeding programs are based upon selection on populations. We tested two restriction enzymes for their efficiency in complexity reduction of the perennial ryegrass genom

SUBMITTER: Byrne S 

PROVIDER: S-EPMC3587605 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature