In Situ probing of newly synthesized peptidoglycan in live bacteria with fluorescent D-amino acids.

ABSTRACT: Tracking a bug's life: Peptidoglycan (PG) of diverse bacteria is labeled by exploiting the tolerance of cells for incorporating different non-natural D-amino acids. These nontoxic D-amino acids preferably label the sites of active PG synthesis, thereby enabling fine spatiotemporal tracking of cell-wall dynamics in phylogenetically and morphologically diverse bacteria. HCC = 7-hydroxycoumarin, NBD = 7-nitrobenzofurazan, TAMRA = carboxytetramethylrhodamine.

SUBMITTER: Kuru E 

PROVIDER: S-EPMC3589519 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature