Improved platelet survival after cold storage by prevention of glycoprotein Ibα clustering in lipid rafts.

ABSTRACT:

Background

Storing platelets for transfusion at room temperature increases the risk of microbial infection and decreases platelet functionality, leading to out-date discard rates of up to 20%. Cold storage may be a better alternative, but this treatment leads to rapid platelet clearance after transfusion, initiated by changes in glycoprotein Ibα, the receptor for von Willebrand factor.

Design and methods

We examined the change in glycoprotein Ibα distribution using Förster resonance energy transfer by time-gated fluorescence lifetime imaging microscopy.

Results

Cold storage induced deglycosylation of glycoprotein Ibα ectodomain, exposing N-acetyl-D-glucosamine residues, which sequestered with GM1 gangliosides in lipid rafts. Raft-associated glycoprotein Ibα formed clu

SUBMITTER: Gitz E 

PROVIDER: S-EPMC3590094 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature