Liquid chromatography-tandem mass spectrometry analysis of human adrenal vein 19-carbon steroids before and after ACTH stimulation.

ABSTRACT:

Context

A broad analysis of adrenal gland-derived 19-carbon (C19) steroids has not been reported. This is the first study that uses liquid chromatography-tandem mass spectrometry to quantify 9 C19 steroids (androgens and their precursors), estrone, and estradiol in the adrenal vein (AV) of women, before and after ACTH stimulation.

Objective

The objective of this study was to define the adrenal androgen metabolome in women before and after ACTH infusion.

Design

This was a retrospective study.

Patients

Seven women, aged 50.4 ± 5.4 years, with suspected diagnosis of an adrenal aldosterone-producing adenoma were included in the study.

Methods

AV and iliac serum samples were collected before and after administration of ACTH (15 minutes). AV samples were analy

SUBMITTER: Rege J 

PROVIDER: S-EPMC3590473 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature