MicroRNA profiling of peripheral nerve sheath tumours identifies miR-29c as a tumour suppressor gene involved in tumour progression.

ABSTRACT:

Background

Neurofibromatosis type 1 is one of the most common familial diseases, the hallmark of which is the development of multiple neurofibromas. These are benign nerve sheath tumours, which can transform into malignant peripheral nerve sheath tumours (MPNST).

Methods

The aim of this study was to identify differentially expressed microRNA (miRNA) in neurofibromas and MPNST obtained from patients with neurofibromatosis type 1 using microarray analysis. Differential expression was validated by reverse transcription quantitative-PCR, and functional studies were performed after transfection of miRNA oligonucleotide mimics into MPNST cells.

Results

Sixteen miRNA were significantly differentially expressed in MPNST compared with NF, and of these fourteen were downregulat

SUBMITTER: Presneau N 

PROVIDER: S-EPMC3590650 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature