Circulating microRNAs as biomarkers for early detection of diffuse-type gastric cancer using a mouse model.

ABSTRACT:

Background

Diffuse-type gastric cancer (DGC) exhibits rapid disease progression and a poor prognosis. There are no effective serum biomarkers for early detection of DGC. We have established an E-cadherin/p53 double conditional knockout (DCKO) mouse line that recapitulates human DGC morphologically and molecularly. In this study we tried to identify circulating microRNAs (miRNAs) as non-invasive biomarkers for DGC diagnosis using DCKO mice.

Methods

We performed miRNA microarray and quantitative reverse transcription-PCR analyses of tissue and serum samples from DCKO mice with DGC and age-matched littermate controls.

Results

Comparative analyses showed that mouse and human primary gastric cancers have similar miRNA expression patterns. Next, we selected some candidate m

SUBMITTER: Rotkrua P 

PROVIDER: S-EPMC3590667 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature