Additions to the Human Plasma Proteome via a Tandem MARS Depletion iTRAQ-Based Workflow.

ABSTRACT: Robust platforms for determining differentially expressed proteins in biomarker and discovery studies using human plasma are of great interest. While increased depth in proteome coverage is desirable, it is associated with costs of experimental time due to necessary sample fractionation. We evaluated a robust quantitative proteomics workflow for its ability (1) to provide increased depth in plasma proteome coverage and (2) to give statistical insight useful for establishing differentially expressed plasma proteins. The workflow involves dual-stage immunodepletion on a multiple affinity removal system (MARS) column, iTRAQ tagging, offline strong-cation exchange chromatography, and liquid chromatography tandem mass spectrometry (LC-MS/MS). Independent workflow experiments were performed in t

SUBMITTER: Cao Z 

PROVIDER: S-EPMC3590782 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature