Broader utilization of origins of DNA replication in cancer cell lines along a 78 kb region of human chromosome 2q34.

ABSTRACT: Human DNA replication depends on the activation of thousands of origins distributed within the genome. The actual distribution of origins is not known, nor whether this distribution is unique to a cell type, or if it changes with the proliferative state of the cell. In this study, we have employed a real-time PCR-based nascent strand DNA abundance assay, to determine the location of origins along a 78 kb region on Chr2q34. Preliminary studies using nascent DNA strands isolated from either HeLa and normal skin fibroblast cells showed that in both cell lines peaks of high origin activity mapped in similar locations. However, the overall origin profile in HeLa cells corresponded to broad origin activation zones, whereas in fibroblasts a more punctuated profile of origin activation was observe

SUBMITTER: Valenzuela MS 

PROVIDER: S-EPMC3590909 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature