Evaluation of 22q11.2 deletion in Cleft Palate patients.

ABSTRACT:

Background

Cleft palate is the commonest multifactorial epigenetic disorder with a prevalence of 0.43-2.45 per 1000. The objectives of this study were to evaluate the clinical features and identify the 22q11.2 deletion in patients with cleft palate in Sri Lanka.

Materials and methods

Cleft patients attending a Teaching Hospital in Sri Lanka were recruited for this study. The relevant data were obtained from review of case notes, interviews, and examination of patients according to a standard evaluation sheet. Quantitative multiplex polymerase chain reaction (PCR) was performed to identify the 22q11.2 deletion. A gel documentation system (Bio-Doc) was used to quantify the PCR product following electrophoresis on 0.8% agarose gel.

Results and conclusion

There were 162 c

SUBMITTER: Prabodha LB 

PROVIDER: S-EPMC3591068 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature