Measurements of the impact of 3' end sequences on gene expression reveal wide range and sequence dependent effects.

ABSTRACT: A full understanding of gene regulation requires an understanding of the contributions that the various regulatory regions have on gene expression. Although it is well established that sequences downstream of the main promoter can affect expression, our understanding of the scale of this effect and how it is encoded in the DNA is limited. Here, to measure the effect of native S. cerevisiae 3' end sequences on expression, we constructed a library of 85 fluorescent reporter strains that differ only in their 3' end region. Notably, despite being driven by the same strong promoter, our library spans a continuous twelve-fold range of expression values. These measurements correlate with endogenous mRNA levels, suggesting that the 3' end contributes to constitutive differences in mRNA levels. We

SUBMITTER: Shalem O 

PROVIDER: S-EPMC3591272 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature