Evaluating primers for profiling anaerobic ammonia oxidizing bacteria within freshwater environments.

ABSTRACT: Anaerobic ammonia oxidizing (anammox) bacteria play an important role in transforming ammonium to nitrogen gas and contribute to fixed nitrogen losses in freshwater environments. Understanding the diversity and abundance of anammox bacteria requires reliable molecular tools, and these are not yet well established for these important Planctomycetes. To help validate PCR primers for the detection of anammox bacteria within freshwater ecosystems, we analyzed representative positive controls and selected samples from Grand River and groundwater sites, both from Ontario, Canada. The objectives of this study were to identify a suitable anammox denaturing gradient gel electrophoresis (DGGE) fingerprint method by using GC-clamp modifications to existing primers, and to verify the specificity of an

SUBMITTER: Sonthiphand P 

PROVIDER: S-EPMC3591393 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature