Roles of the Y-family DNA polymerase Dbh in accurate replication of the Sulfolobus genome at high temperature.

ABSTRACT: The intrinsically thermostable Y-family DNA polymerases of Sulfolobus spp. have revealed detailed three-dimensional structure and catalytic mechanisms of trans-lesion DNA polymerases, yet their functions in maintaining their native genomes remain largely unexplored. To identify functions of the Y-family DNA polymerase Dbh in replicating the Sulfolobus genome under extreme conditions, we disrupted the dbh gene in Sulfolobus acidocaldarius and characterized the resulting mutant strains phenotypically. Disruption of dbh did not cause any obvious growth defect, sensitivity to any of several DNA-damaging agents, or change in overall rate of spontaneous mutation at a well-characterized target gene. Loss of dbh did, however, cause significant changes in the spectrum of spontaneous forward mutatio

SUBMITTER: Sakofsky CJ 

PROVIDER: S-EPMC3591481 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature