Molecular fingerprinting of cyanobacteria from river biofilms as a water quality monitoring tool.

ABSTRACT: Benthic cyanobacterial communities from Guadarrama River (Spain) biofilms were examined using temperature gradient gel electrophoresis (TGGE), comparing the results with microscopic analyses of field-fixed samples and the genetic characterization of cultured isolates from the river. Changes in the structure and composition of cyanobacterial communities and their possible association with eutrophication in the river downstream were studied by examining complex TGGE patterns, band extraction, and subsequent sequencing of 16S rRNA gene fragments. Band profiles differed among sampling sites depending on differences in water quality. The results showed that TGGE band richness decreased in a downstream direction, and there was a clear clustering of phylotypes on the basis of their origins from d

SUBMITTER: Loza V 

PROVIDER: S-EPMC3591963 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature