Development of a new generation of vectors for gene expression, gene replacement, and protein-protein interaction studies in mycobacteria.

ABSTRACT: Escherichia coli-mycobacterium shuttle vectors are important tools for gene expression and gene replacement in mycobacteria. However, most of the currently available vectors are limited in their use because of the lack of extended multiple cloning sites (MCSs) and convenience of appending an epitope tag(s) to the cloned open reading frames (ORFs). Here we report a new series of vectors that allow for the constitutive and regulatable expression of proteins, appended with peptide tag sequences at their N and C termini, respectively. The applicability of these vectors is demonstrated by the constitutive and induced expression of the Mycobacterium tuberculosis pknK gene, coding for protein kinase K, a serine-threonine protein kinase. Furthermore, a suicide plasmid with expanded MCS for creatin

SUBMITTER: Parikh A 

PROVIDER: S-EPMC3591980 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature