Isolation of a substantial proportion of forest soil bacterial communities detected via pyrotag sequencing.

ABSTRACT: We isolated 1,264 bacterial strains from forest soils previously surveyed via pyrosequencing of rRNA gene amplicons. Conventional culturing techniques recovered a substantial proportion of the community, with isolates representing 22% of 98,557 total pyrotags. Growth characteristics of isolates indicated that ecological traits were associated with relative abundances of corresponding pyrotag operational taxonomic units.

SUBMITTER: VanInsberghe D 

PROVIDER: S-EPMC3592214 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature