DJ-1 regulates mast cell activation and IgE-mediated allergic responses.

ABSTRACT:

Background

DJ-1 is an antioxidant protein known to reduce levels of reactive oxygen species (ROS), but its presence or function in mast cells and allergic diseases is unknown.

Objectives

We sought to determine the role and mechanism of DJ-1 in allergic responses in vitro and in vivo.

Methods

ROS and DJ-1 levels in serum or culture medium were measured with ELISA kits. The role of DJ-1 was evaluated in mast cell cultures and passive cutaneous anaphylaxis in normal or DJ-1 knockout (KO) mice. The mechanism of DJ-1 action was examined by using immunoblotting, immunoprecipitation, RT-PCR, and other molecular biological approaches.

Results

Patients with atopic dermatitis had increased levels of ROS and diminished levels of DJ-1. DJ-1 KO mice exhibited enhanced passi

SUBMITTER: Kim DK 

PROVIDER: S-EPMC3594621 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature